It depends. I have been penalized for negotiating as well as have been rewarded. I do remember distinctly irritating a hiring manager when I responded to her inquiry of "how much do you want to make" with "whatever is the prevailing market rate is" She wanted me to give her a number and I politely played hard ball not wanting to undersell myself. Anyway the hiring manager decided that approach made me difficult.

Women are often penalized for not being "nice" in the workplace as well. It may happen in the hiring process or show up later in performance reviews. It is a delicate dance of negotiating, being strong and being "non-threatening."