Yes. Definitely. Absolutely. The users will perceive the difference. This is a slowdown of over 3X. If you're loading resources (in a variety of synchronous batches of asynchronous requests) for your web application, these slowdowns compound significantly.
Of course, the design of the web application plays a huge role, but assuming the worst (every user action triggers a request), a 3X slowdown is perceived by a variety of users.