Kudos on Opps Daily, big fan here, it's great!
I will say that It's been a valuable exercise to e-mail every unsubscriber and ask why they left.
The number one reason people leave is because they don't want to get an e-mail every day. We now offer a weekly recap email because of this feedback, and I've actually had people return to the list who had previously left.
Sending an e-mail to someone is a privilege. Especially a daily e-mail. In my opinion our time/attention is one of the most precious things we have.
I want the reader to be able to read and comprehend the opportunity in under 30 seconds and leave feeling like the e-mail had a positive impact on their day.
If you're looking for any advice in specific, get in touch, I'm always happy to help :)