Not if your HOSTS file is >135KB (the one you've provided is 373KB), you're using Windows 8 or earlier and you haven't disabled the DNS Client service.
http://winhelp2002.mvps.org/hostswin8.htm (about half way down)
FWIW I do the same, also use that host file at the border router and yes the difference is quite big. I'm always shocked about the extra adverts I see when using a computer or tablet outside of my own network.
I've never had an issue on XP or 7 and I've used the same host file shared above with many, many more that I've personally added (my hosts file is nearly 500KB)