Another lecture which was memorable dealt with the sheer variety of different situations and biomechanisms which you can rule out using the Second Law of Thermodynamics. The bottom line is that when you're at thermal equilibrium then everything must jitter thermally by the same amount, which is what excludes "Brownian ratchets" [1] from working: if you peek inside at the spring and asymmetrical "teeth" inside an actual ratchet, then you realize that the work extracted actually comes from heat-transfer to the spring, which is being implicitly assumed to be at 0 temperature in the naive "oh, you get energy out of nothing!" argument. It's a very simple 2nd-law argument to make, but really hard to poke around inside and figure out what's going on internally.