Depends on the branch. In quantitative finance: C++, R, F#, Q, OCaml, MATLAB, Python (NumPy and SciPy)
EDIT: As for the GUI comment, I am actually trying to stay away from a bloated GUI as speed is key in this business.
Also, when we, as a program, went to Chicago during late October, so many people said that C# was becoming an important language to know if you plan on doing quant work. Up until that point, I had been focused primarily on C and C++. I thought it would be wise to do some work with C# if people at quant shops were telling me this is something I need to know.